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KMID : 1134820110400101397
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Journal of the Korean Society of Food Science and Nutrition
2011 Volume.40 No. 10 p.1397 ~ p.1403
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Biological Activities of Isolated Icariin from Epimedium koreanum Nakai
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Kim Seo-Jin
Park Myoung-Su
Tian Ding
Jun Wang
Oh Deog-Hwan
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Abstract
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Epimedium koreanum Nakai is a wild medicinal plant commonly consumed in South Korea due to its health beneficial effects. In the present study, the antioxidative, antimutagenic and immunological activities of E. koreanum Nakai extracts were investigated for their use in food. The yields of icariin compounds from the ethanol extract as well as the ethyl acetate, butanol, hexane, water, and chloroform fractions of E. koreanum were 27.9, 2.5, 1.7, 1.4, and 1.3 μg/g, respectively. The icariin components (295.5 μg/g) were collected from the ethyl acetate fraction by thin layer chromatography (TLC) and analyzed via high performance liquid chromatography (HPLC). The antioxidant activities of each fraction were as follows: ethyl acetate (49.0 μg/mL), butanol (59.2 μg/mL), hexane (119.8 μg/mL), water (122.0 μg/mL), and chloroform (138.5 μg/mL), based on RC50 μg/mL. Icariin, isolated and identified as the main component, showed strong antioxidant activity with a RC50 value of 15.3 μg/mL, which was higher than those of ascorbic acid (19.5 μg/mL) and α-tocopherol (18.2 μg/mL). In an Ames test, none of the fractions produced mutagenic effects on Salmonella Typhimurium TA98 and TA100. In an immunomodulating activity test, the effects of E. koreanum Nakai on B cells (Rhamos) and T cells (Jurkat) were investigated. These results show that the growth and viability of B and T cells were increased by isolated icariin components for 1.27 and 1.28 fold, respectively. These results also provide preliminary data for the development of E. koreanum Nakai as an edible food material.
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KEYWORD
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Epimedium koreanum Nakai, icariin, DPPH, Ames test, MTT assay
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